by MyH Editorial Team | Oct 9, 2023 | Lab Protocols, Red Blood Cells
Procedure At A Glance Perls’ Prussian blue stain is used to histochemically detect and visualize ferric iron (Fe3+) deposits in cells and tissues. Fix slides in absolute methanol for 10 minutes and dry. Submerge slides in the working solution (2% potassium...
by MyH Editorial Team | Oct 9, 2023 | Lab Protocols, Red Blood Cells
Procedure At-A-Glance The Leishman stain is used in microscopy to differentiate types of blood cells and to detect blood parasites in peripheral blood smears. Flood Slide Protocol Prepare 0.066 M phosphate buffer at pH 6.8. Cover the smear with Leishman stain. Wait...
by MyH Editorial Team | Oct 9, 2023 | Red Blood Cells
Key Takeaways Megaloblastic anemia is caused by faulty DNA synthesis in red cell precursors, almost always due to vitamin B12 (cobalamin) or folate (vitamin B9) deficiency, producing oversized red cells (macrocytes) with a mean corpuscular volume typically above 100...
by MyH Editorial Team | Oct 9, 2023 | Red Blood Cells
Key Takeaways Iron deficiency anemia is the most common nutritional deficiency worldwide. It develops when the body lacks the iron necessary to produce adequate hemoglobin, causing red blood cells to become smaller, paler, and less capable of carrying oxygen to...
by MyH Editorial Team | Oct 9, 2023 | Red Blood Cells
Key Takeaways Beta-thalassemia is an inherited disorder caused by mutations in the HBB gene that reduce or stop production of the beta-globin chain of hemoglobin. The resulting imbalance between alpha and beta chains drives ineffective red cell production, ongoing red...
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