Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Lab Protocols
Serologic Crossmatching
Crossmatching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.
Flow Cytometry Immunophenotyping of Blood
Blood cells stained with fluorescent antibodies reveal hidden markers, like a cellular fingerprint. Flow cytometry analyzes millions of cells, painting a detailed picture of immune health, disease clues, and treatment insights.
JAK2 V617F Mutation ARMS PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Serologic Crossmatching
Crossmatching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.
Agarose Gel Electrophoresis Preparation for PCR
Agarose gel electrophoresis: where we separate and visualize amplified DNA fragments.
Hematopoietic cell surface markers
Surface markers, also known as clusters of differentiation (CDs), act like identifying badges for different types of white blood cells.
Agarose Gel Electrophoresis Preparation for PCR
Agarose gel electrophoresis: where we separate and visualize amplified DNA fragments.
Hb S Sickling Test
This test unmasks sickle cells: crescent shadows under the microscope, revealing HbS’s hidden grip on red blood cells.
Indirect Antiglobulin (Coombs) Test (IAT)
Unmask hidden blood threats! IAT protocol detects antibodies in serum, ensuring safe transfusions & diagnosing immune disorders.
Direct Antiglobulin (Coombs) Test (DAT)
To detect the presence of antibodies or complement proteins bound to red blood cells (RBCs) in vivo, indicating potential immune-mediated hemolysis.
Rh Phenotyping using Tube Method
Unraveling the Rh code! Mix blood with anti-sera in tubes. Incubate, centrifuge, & observe. Clumping reveals antigen presence, guiding safe transfusions.
