by MH Team | Nov 14, 2023 | Lab Protocols, Red Blood Cells
Procedure-at-a-Glance StageActivity1. PreparationReagent thawing & Workstation UV sterilization2. Master MixCombining Polymerase, dNTPs, Buffers, and Primers3. Amplification30 cycles of Denaturation, Annealing, and Extension4. VerificationGel Electrophoresis (1%...
by MH Team | Nov 13, 2023 | Transfusion Medicine
Key Takeaways ABO blood group is the most important blood group system in transfusion and organ transplantation medicine. ABO blood group antigens can be found on red cells, white cells, platelets and many circulating proteins. Transfusion incompatibility can...
by MH Team | Nov 9, 2023 | Lab Protocols, White Blood Cells
Procedure-at-a-Glance StepActionDuration1. FixationFix fresh smears in Cold Formalin-Methanol (0-10°C).30 Seconds2. WashingGently rinse with running tap water and air dry.—3. IncubationImmerse in freshly prepared buffered substrate.15–30 Minutes4....
by MH Team | Nov 9, 2023 | Red Blood Cells
Key Takeaways Aplastic anemia (AA) causes pancytopenia (reduction of all blood cell types: RBCs, WBCs and platelets) due to aplasia (failure) of the bone marrow to function (to produce blood cells). Causes▾: Congenital e.g. Fanconi anemia, dyskeratosis...
by MH Team | Nov 3, 2023 | Platelet Disorders
Key Takeaways Thrombotic thrombocytopenic purpura (TTP) is a type of thrombocytopenia due to deficiency of ADAMTS13 metalloprotease which leads to formation of platelet thrombi in microvessels. Signs and symptoms (pentad) ▾: Thrombocytopenia...
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