by MH Team | Dec 27, 2023 | Platelet Disorders
TL;DR Venous thromboembolism or also known as VTE is a condition where a blood clot (thrombosis) forms in a deep vein, usually in the leg (deep vein thrombosis) or pelvis, and can travel to the lungs, causing a pulmonary embolism. This is a serious condition that can...
by MH Team | Dec 22, 2023 | Platelet Disorders
TL;DR Arterial thrombosis forms when a clot blocks an artery, cutting off blood supply to vital organs. Risk factors ▾: Age, smoking, high cholesterol, diabetes, and others. Symptoms ▾: Sudden pain, weakness, numbness, and discoloration can signal a...
by MH Team | Dec 21, 2023 | Lab Protocols, Transfusion Medicine
Procedure At A Glance The purpose of Rh typing is to determine the presence or absence of the RhD antigen on an individual’s red blood cells to prevent transfusion reactions and hemolytic disease of the newborn (HDN). Label tubes. Add a drop of a 2-5% patient...
by MH Team | Dec 20, 2023 | Lab Protocols, White Blood Cells
Procedure At A Glance Total Time of IHC Staining: ~4–6 Hours (excluding overnight incubation) Deparaffinize: Xylene (3 changes, 10 mins each) → Rehydrate in graded Alcohols (100% to 50%) → Water. Antigen Retrieval: Heat slides in Citrate Buffer...
by MH Team | Dec 19, 2023 | Lab Protocols, Platelet Disorders
Procedure-at-a-Glance Processing: Spin 1 (Slow): Centrifuge whole blood at ~200g for 10-15 mins to obtain Platelet Rich Plasma (PRP). Spin 2 (Fast): Centrifuge remaining blood at ~2000g for 15-20 mins to obtain Platelet Poor Plasma (PPP) (used as the...
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