by MyH Editorial Team | Dec 20, 2023 | Lab Protocols, White Blood Cells
Procedure At A Glance Total Time of IHC Staining: ~4–6 Hours (excluding overnight incubation) Deparaffinize: Xylene (3 changes, 10 mins each) → Rehydrate in graded Alcohols (100% to 50%) → Water. Antigen Retrieval: Heat slides in Citrate Buffer...
by MyH Editorial Team | Dec 19, 2023 | Hemostasis Disorders, Lab Protocols
Procedure-at-a-Glance Processing: Spin 1 (Slow): Centrifuge whole blood at ~200g for 10-15 mins to obtain Platelet Rich Plasma (PRP). Spin 2 (Fast): Centrifuge remaining blood at ~2000g for 15-20 mins to obtain Platelet Poor Plasma (PPP) (used as the...
by MyH Editorial Team | Dec 18, 2023 | Hemostasis Disorders
Key Takeaways Hemostasis is the intricate process of stopping and restarting blood flow after an injury. It has different stages, each playing a crucial role and relying on the previous one for successful completion. Vascular Spasm ▾: The immediate constriction...
by MyH Editorial Team | Dec 17, 2023 | Hemostasis Disorders
Key Takeaways Platelet or a thrombocyte is a small, anucleated cellular fragment derived from bone marrow megakaryocyte. Lifespan & Count: They circulate for 7–10 days with a normal reference range of 150,000 – 450,000/µL. Morphology ▾:...
by MyH Editorial Team | Dec 14, 2023 | White Blood Cells
Key Takeaways Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis, resulting in peripheral blood cytopenias and a high risk of progression to Acute Myeloid Leukemia (AML). Peak...
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