Lab Protocols

Bone Marrow Aspiration and Trephine Biopsy

An overview of bone marrow aspiration and trephine biopsy. This guide covers key indications, procedural best practices, and modern result interpretation.

Wright Stain for Peripheral Blood Smear

Learn the Wright stain principle, step-by-step protocol, interpretation and troubleshooting tips in this guide for peripheral blood smears.

Coagulation Screening Panel Interpretation (Coagulation Panel)

A coagulation screening panel is a vital diagnostic tool. It assesses blood clotting (hemostasis) via PT, aPTT, & platelet count, revealing bleeding or clotting risks.

Iron studies reveal iron deficiency, overload, and other conditions. Learn how MCV, ferritin, and other markers help decipher iron status and guide treatment.

This image showcases the distinct bands representing successful amplification of beta-globin gene for downstream sequencing protocol.

Agarose Gel Electrophoresis Preparation for PCR

Lab Protocols, Red Blood Cells

Agarose gel electrophoresis: where we separate and visualize amplified DNA fragments.

Panel A reveals healthy red blood cells, while Panel B showcases the abnormal cell shapes associated with sickle cell disease in a sickling test.

Sickle Cell Disease Test

Lab Protocols, Red Blood Cells

Explore the Sickle Cell Disease Test: a vital lab protocol for detecting sickle cell disease. Learn its principle, procedure, and result interpretation.

Indirect Antiglobulin (Coombs) Test (IAT)

Lab Protocols, Transfusion Medicine

Indirect Coombs test (IAT) procedure detects antibodies in serum, ensuring safe transfusions & diagnosing immune disorders.

Direct Antiglobulin (Coombs) Test (DAT)

Lab Protocols, Transfusion Medicine

To detect the presence of antibodies or complement proteins bound to red blood cells (RBCs) in vivo, indicating potential immune-mediated hemolysis.

This image depicts the red cell agglutination test score for forward blood group typing, a crucial step in determining an individual's ABO blood type. The agglutination patterns observed in the test tubes provide clear and distinct indicators of the blood group, ensuring accurate blood compatibility assessments for transfusions.

Rh Typing using Tube Method

Lab Protocols, Transfusion Medicine

Unraveling the Rh code! Mix blood with anti-sera in tubes. Incubate, centrifuge, & observe. Clumping reveals antigen presence, guiding safe transfusions.

Microscopic image of a bone marrow trephine biopsy showcasing a CD3-positive T lymphocyte (brown stain) amidst other blood cells.

IHC – Immunohistochemistry Staining

Lab Protocols, White Blood Cells

This immunohistochemistry protocol guides you step-by-step through visualizing specific proteins in tissue sections, aiding in accurate diagnosis of various diseases.

Bone Marrow Aspiration and Trephine Biopsy

Wright Stain for Peripheral Blood Smear

Coagulation Screening Panel Interpretation (Coagulation Panel)

Iron Studies Interpretation: The Highs and Lows

Blood Collection Tube (Vacutainer) Colors & Types

Interpretation of Antibody Identification Antigram

Phlebotomy Procedure: Best Practices & Venipuncture

Preparation of Thick Blood Films for Parasites

Iron Studies Interpretation: The Highs and Lows

Agarose Gel Electrophoresis Preparation for PCR

Sickle Cell Disease Test

Indirect Antiglobulin (Coombs) Test (IAT)

Direct Antiglobulin (Coombs) Test (DAT)

Rh Typing using Tube Method

IHC – Immunohistochemistry Staining

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