Lab Protocols

Wright-Giemsa Stain

Master the Wright-Giemsa stain: a complete guide to principles, protocols, and interpreting peripheral blood and bone marrow smears.

Bone Marrow Aspiration and Trephine Biopsy

An overview of bone marrow aspiration and trephine biopsy. This guide covers key indications, procedural best practices, and modern result interpretation.

Wright Stain for Peripheral Blood Smear

Learn the Wright stain principle, step-by-step protocol, interpretation and troubleshooting tips in this guide for peripheral blood smears.

Blood collection tubes come in various colors for specific tests. Red & gold tubes separate serum, while purple & pink tubes prevent clotting for blood cell analysis. Choose the right tube for accurate results!

Normal G6PD activity: Bright green fluorescence. Intermediate G6PD activity: Reduced or yellowish fluorescence. No or very weak fluorescence.

Glucose-6-Phosphate Dehydrogenase (G6PD) Fluorescent Spot Test (G6PD Test)

Lab Protocols, Red Blood Cells

The G6PD fluorescent spot test is a rapid and simple screening method for G6PD deficiency that utilizes the enzyme’s ability to reduce NADP+ to NADPH.

Schematic Diagram of the Beta-Globin Gene and PCR Primer Locations: A detailed illustration of the beta-globin gene structure and the positions of primers used for targeted amplification of specific regions.

Conventional PCR Protocol for Beta Thalassemia Downstream Sequencing

Lab Protocols, Red Blood Cells

The beta globin gene PCR protocol for sequencing involves amplifying the beta globin gene using specific primers, followed by Sanger sequencing to determine the DNA sequence.

NAP Scoring System for Peripheral Blood Smear Evaluation - Illustrates the NAP scoring system used to quantify neutrophils - whether they are mainly mature or immature cells.

Leukocyte/Neutrophil Alkaline Phosphatase (LAP/NAP) Stain

Lab Protocols, White Blood Cells

The NAP stain is used to differentiate between granulocytes and agranulocytes based on their naphthol AS-D chloroacetate esterase (NASD-CE) enzyme activity.

Periodic Acid Schiff Stain (PAS Staining)

Lab Protocols, White Blood Cells

PAS stain is a histochemical technique that utilizes periodic acid-Schiff (PAS) reagent to detect and visualize carbohydrate-rich structures in cells and tissues, such as glycogen, glycoproteins, and mucins.