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Lab Protocols
Serologic Crossmatching (Blood Compatibility Test)
Cross-matching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.
Flow Cytometry Immunophenotyping of Blood
Blood cells stained with fluorescent antibodies reveal hidden markers, like a cellular fingerprint. Flow cytometry analyzes millions of cells, painting a detailed picture of immune health, disease clues, and treatment insights.
Interpretation of Antibody Identification Antigram
Antigen identification antigrams decode antibody reactions in blood transfusions, ensuring safe blood selection by identifying compatible red blood cells that lack the antigen targeted by the recipient’s antibody.
Antibody Identification
Incubate patient serum with panel RBCs, check for agglutination! Enzymes & antiglobulin tests may be used to reveal hidden antibody reactions.
Antibody Identification
Incubate patient serum with panel RBCs, check for agglutination! Enzymes & antiglobulin tests may be used to reveal hidden antibody reactions.
Manual APTT Mixing Study
Manual APTT mixing studies involve mixing patient plasma with normal plasma or specific factor concentrates to differentiate factor deficiencies from inhibitors
ABO RhD Test (Blood Group Typing) Tube Method
The ABO RhD Blood Grouping Tube Method is a serological technique used to determine an individual’s blood type by mixing blood serum and red blood cells with antisera and observing for agglutination.
High Performance Liquid Chromatography (HPLC) for Hemoglobinopathies Screening
HPLC for hemoglobin subtype identification separates and quantifies different hemoglobin variants based on their unique chromatographic properties.
Glucose-6-Phosphate Dehydrogenase (G6PD) Fluorescent Spot Test (G6PD Test)
The G6PD fluorescent spot test is a rapid and simple screening method for G6PD deficiency that utilizes the enzyme’s ability to reduce NADP+ to NADPH.
Conventional PCR Protocol for Beta Thalassemia Downstream Sequencing
The beta globin gene PCR protocol for sequencing involves amplifying the beta globin gene using specific primers, followed by Sanger sequencing to determine the DNA sequence.
Leukocyte/Neutrophil Alkaline Phosphatase (LAP/NAP) Stain
The NAP stain is used to differentiate between granulocytes and agranulocytes based on their naphthol AS-D chloroacetate esterase (NASD-CE) enzyme activity.