Lab Protocols | My Hematology

Lab Protocols

Serologic Crossmatching

Crossmatching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.

Flow Cytometry Immunophenotyping of Blood

Blood cells stained with fluorescent antibodies reveal hidden markers, like a cellular fingerprint. Flow cytometry analyzes millions of cells, painting a detailed picture of immune health, disease clues, and treatment insights.

Hematopoietic cell surface markers

Surface markers, also known as clusters of differentiation (CDs), act like identifying badges for different types of white blood cells.

ABO RhD Blood Grouping Tube Method

Lab Protocols, Transfusion Medicine

The ABO RhD Blood Grouping Tube Method is a serological technique used to determine an individual’s blood type by mixing blood serum and red blood cells with antisera and observing for agglutination.

High Performance Liquid Chromatography (HPLC) for Identification of Hemoglobin Subtypes

Lab Protocols, Red Blood Cells

HPLC for hemoglobin subtype identification separates and quantifies different hemoglobin variants based on their unique chromatographic properties.

Glucose-6-Phosphate Dehydrogenase (G6PD) Fluorescent Spot Test

Lab Protocols, Red Blood Cells

The G6PD fluorescent spot test is a rapid and simple screening method for G6PD deficiency that utilizes the enzyme’s ability to reduce NADP+ to NADPH.

Immunohistochemistry Staining (IHC)

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Immunohistochemistry Staining (IHC)

Lab Protocols, White Blood Cells

This immunohistochemistry protocol guides you step-by-step through visualizing specific proteins in tissue sections, aiding in accurate diagnosis of various diseases.

Platelet Aggregation Test (PAT)

Platelet Aggregation Test (PAT)

Lab Protocols, Platelet Disorders

Platelet aggregation test: Checking how blood cells clump to form clots using different agonists.

Immunohistochemistry Staining (IHC)

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Immunohistochemistry Staining (IHC)

Lab Protocols, White Blood Cells

This immunohistochemistry protocol guides you step-by-step through visualizing specific proteins in tissue sections, aiding in accurate diagnosis of various diseases.

Leishman Stain

Leishman Stain

Lab Protocols, Red Blood Cells

Leishman stain is used commonly for the identification of different cells present in the peripheral blood smear. It has acidic and basic properties.