Agarose gel electrophoresis: where we separate and visualize amplified DNA fragments.
Lab Protocols
Sickle Cell Disease Test
Explore the Sickle Cell Disease Test: a vital lab protocol for detecting sickle cell disease. Learn its principle, procedure, and result interpretation.
Indirect Antiglobulin (Coombs) Test (IAT)
Indirect Coombs test (IAT) procedure detects antibodies in serum, ensuring safe transfusions & diagnosing immune disorders.
JAK2 V617F Mutation Allele Specific PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Serologic Crossmatching (Blood Compatibility Test)
Cross-matching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.
JAK2 V617F Mutation Allele Specific PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Activated Partial Thromboplastin Time (aPTT) Blood Test
The APTT assay measures how long it takes for a blood clot to form by measuring the amount of time it takes for fibrinogen to be converted to fibrin.
Prothrombin Time (PT) & INR
The PT assay is used to diagnose and monitor a variety of bleeding and clotting disorders by measuring how long it takes a blood clot to form.
Erythrocyte sedimentation rate (ESR)
ESR measures the sedimentation rate or the length red blood cells fall in a vertical tube over a period of time.
Brilliant Cresyl Blue (BCB) Stain (Methylene Blue)
BCB or new methylene blue (NMB) stains are also known as supravital stains. These stains are commonly used to stain reticulocytes, Heinz bodies or H inclusions
Preparation of Peripheral Blood Smears
A drop of blood spread on a slide, reveals morphological abnormalities of the blood cells that can be viewed under the microscope.
CBC with Differential and Other Reference Ranges
Reference ranges like complete blood count (CBC) with differential are important because they provide a baseline for interpreting laboratory test results.