Explore hematopoietic cell markers & CD34: identification, functions, flow cytometry, and clinical uses in hematology and leukemia.
Lab Protocols
Agarose Gel Electrophoresis Preparation for PCR
Agarose gel electrophoresis: where we separate and visualize amplified DNA fragments.
Sickle Cell Disease Test
Explore the Sickle Cell Disease Test: a vital lab protocol for detecting sickle cell disease. Learn its principle, procedure, and result interpretation.
Antibody Screening
Antibody screening mixes patient plasma with red blood cells to detect unexpected antibodies. Agglutination indicates possible antibodies, requiring further identification for safe blood transfusions
JAK2 V617F Mutation Allele Specific PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Antibody Screening
Antibody screening mixes patient plasma with red blood cells to detect unexpected antibodies. Agglutination indicates possible antibodies, requiring further identification for safe blood transfusions
Periodic Acid Schiff Stain (PAS Staining)
PAS stain is a histochemical technique that utilizes periodic acid-Schiff (PAS) reagent to detect and visualize carbohydrate-rich structures in cells and tissues, such as glycogen, glycoproteins, and mucins.
Activated Partial Thromboplastin Time (aPTT) Blood Test
The APTT assay measures how long it takes for a blood clot to form by measuring the amount of time it takes for fibrinogen to be converted to fibrin.
Prothrombin Time (PT) & INR
The PT assay is used to diagnose and monitor a variety of bleeding and clotting disorders by measuring how long it takes a blood clot to form.
Erythrocyte Sedimentation Rate or ESR
ESR measures the sedimentation rate or the length red blood cells fall in a vertical tube over a period of time.
Supravital Stain: BCB or NMB
BCB or new methylene blue (NMB) stains are also known as supravital stains. These stains are commonly used to stain reticulocytes, Heinz bodies or H inclusions
Preparation of Peripheral Blood Smears
A drop of blood spread on a slide, reveals morphological abnormalities of the blood cells that can be viewed under the microscope.