Incubate patient serum with panel RBCs, check for agglutination! Enzymes & antiglobulin tests may be used to reveal hidden antibody reactions.
Lab Protocols
Antibody Screening
Antibody screening mixes patient plasma with red blood cells to detect unexpected antibodies. Agglutination indicates possible antibodies, requiring further identification for safe blood transfusions
JAK2 V617F Mutation Allele Specific PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Blood Collection Tube (Vacutainer) Types
Blood collection tubes come in various colors for specific tests. Red & gold tubes separate serum, while purple & pink tubes prevent clotting for blood cell analysis. Choose the right tube for accurate results!
Interpretation of Antibody Identification Antigram
Antigen identification antigrams decode antibody reactions in blood transfusions, ensuring safe blood selection by identifying compatible red blood cells that lack the antigen targeted by the recipient’s antibody.
Antibody Identification
Incubate patient serum with panel RBCs, check for agglutination! Enzymes & antiglobulin tests may be used to reveal hidden antibody reactions.
Direct Antiglobulin (Coombs) Test (DAT)
To detect the presence of antibodies or complement proteins bound to red blood cells (RBCs) in vivo, indicating potential immune-mediated hemolysis.
Rh Typing using Tube Method
Unraveling the Rh code! Mix blood with anti-sera in tubes. Incubate, centrifuge, & observe. Clumping reveals antigen presence, guiding safe transfusions.
Immunohistochemistry Staining (IHC)
This immunohistochemistry protocol guides you step-by-step through visualizing specific proteins in tissue sections, aiding in accurate diagnosis of various diseases.
Platelet Aggregation Test (PAT)
Platelet aggregation test: Checking how blood cells clump to form clots using different agonists.
Hemolysate Preparation for Hb Electrophoresis or Alkaline Denaturation Tests
Red blood cells lysed to release hemoglobin for Hb electrophoresis and alkaline denaturation test. Hemolysate preparation requires careful steps to ensure accuracy and reliability of results.
Manual APTT Mixing Studies
Manual APTT mixing studies involve mixing patient plasma with normal plasma or specific factor concentrates to differentiate factor deficiencies from inhibitors