Lab Protocols
Phlebotomy Procedure: Best Practices & Venipuncture
Phlebotomy involves drawing blood using a needle from a vein in the arm. To ensure patient comfort and accurate results, a skilled phlebotomist locates a vein, disinfects the area, inserts a needle, collects blood, and then removes the needle and applies pressure.
Interpretation of Antibody Identification Antigram
Antigen identification antigrams decode antibody reactions in blood transfusions, ensuring safe blood selection by identifying compatible red blood cells that lack the antigen targeted by the recipient’s antibody.
Antibody Identification
Incubate patient serum with panel RBCs, check for agglutination! Enzymes & antiglobulin tests may be used to reveal hidden antibody reactions.
Antibody Screening
Antibody screening mixes patient plasma with red blood cells to detect unexpected antibodies. Agglutination indicates possible antibodies, requiring further identification for safe blood transfusions
JAK2 V617F Mutation Allele Specific PCR Protocol
Uncover the JAK2 V617F mutation, a key driver in Myeloproliferative Neoplasms (MPNs). Learn ARMS PCR, a reliable technique for diagnosis!
Serologic Crossmatching (Blood Compatibility Test)
Cross-matching mimics transfusion by mixing recipient serum & donor cells. Clumping (agglutination) indicates incompatibility, preventing transfusion reactions.
Flow Cytometry Immunophenotyping of Blood
Blood cells stained with fluorescent antibodies reveal hidden markers, like a cellular fingerprint. Flow cytometry analyzes millions of cells, painting a detailed picture of immune health, disease clues, and treatment insights.